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吴晓晖 博士

  • 吴晓晖 博士

    常务副所长

    教授

    遗传工程国家重点实验室PI

    遗传与发育协同创新中心PI

     

    电话: 021-65643718

    E-mail: xiaohui_wu@fudan.edu.cn

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    男,1972年生,博士、教授、博导。1990年进入复旦大学遗传学专业本科学习,1994年获准提前攻读复旦大学遗传学研究所研究生,1997-2000年间赴美国耶鲁大学联合培养,1995年和2001年分别获得学士和博士学位。2001年留校到发育生物学研究所工作,历任教师、副教授、教授、博导,2012年起兼任复旦大学附属儿科医院儿童发育与疾病转化医学研究中心双聘PI和复旦大学遗传工程国家重点实验室PI。2015年起担任发育生物学研究所副所长。2016年担任上海市肾脏发育和儿童肾脏病研究中心副主任。目前还担任中国遗传学会理事、青年委员会委员,上海市遗传学会理事,上海市实验动物学会理事,中国细胞生物学会会员。

     

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    小鼠与人基因功能和作用机理保守,器官发育和代谢调控过程相似,对研究发育和疾病机理,发展疾病预防、诊断和治疗方法具有重要价值。本课题组建立了用于小鼠高效基因诱变和功能分析的哺乳动物piggyBac转座子、可见荧光标记和体细胞有丝分裂重组等一系列遗传学研究方法,主持培育了具有5,000多种基因突变体的世界最大小鼠基因突变体库,为进一步解析发育和疾病机理,研发疾病动物模型奠定了良好基础。目前,本课题组正依托这一小鼠突变体资源,结合细胞和生化手段筛选研究代谢和肾脏发育相关基因及作用机理。

     

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    全国高校十大科技进展(2005),上海市科教党委系统青年科技创新人才(2006),上海市青年科技启明星(2006, 2010),973计划项目首席科学家(2006),上海市自然科学一等奖(2007),上海市曙光学者(2009),新世纪百千万人才工程国家级人选(2009),上海市青年科技启明星追踪计划(2010),上海青年科技英才(2010),上海领军人才(2011)等,国务院政府特殊津贴(2013),上海市优秀学术带头人(2015)等。

     

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    1. ADAM10-Initiated Release of Notch Intracellular Domain Regulates Microtubule Stability and Radial Migration of Cortical Neurons. Yang Z, Li PF, Chen RC, Wang J, Wang S, Shen Y, Wu X, Fang B, Cheng X, Xiong ZQ. Cereb Cortex. 2017 Feb 3:1-14. [Epub ahead of print]

    2. L2hgdh deficiency accumulates L-2-hydroxyglutarate with progressive leukoencephalopathy and neurodegeneration. Ma S, Sun R, Jiang B, Gao J, Deng W, Liu P, He R, Cui J, Ji M, Yi W, Yang P, Wu X, Xiong Y, Qiu Z, Ye D, Guan KL. Mol Cell Biol. 2017 Jan 30. [Epub ahead of print]

    3. PKA-RIIB deficiency induces brown fat-like adipocytes in inguinal WAT and promotes energy expenditure in male FVB/NJ mice. Jing S, Wu W, Huang S, Xue R, Wang Y, Wan Y, Zhang L, Qin L, Zhang Q, Zhu X, Zhang Z, Ye H, Wu X, Li Y. Endocrinology. 2016 Dec 14. [Epub ahead of print]

    4. Zfp462 deficiency causes anxiety-like behaviors with excessive self-grooming in mice. Wang B, Zheng Y, Shi H, Du X, Zhang Y, Wei B, Luo M, Wang H, Wu X, Hua X, Sun M, Xu X. Genes Brain Behav. 2017 Feb;16(2):296-307.

    5. Disruption of GPR45 causes reduced hypothalamic POMC expression and obesity. Cui J, Ding Y, Chen S, Zhu X, Wu Y, Zhang M, Zhao Y, Li TR, Sun LV, Zhao S, Zhuang Y, Jia W, Xue L, Han M, Xu T*, Wu X*. J Clin Invest. 2016 Sep 1;126(9):3192-206. (*通讯作者)

    6. Gen1 and Eme1 Play Redundant Roles in DNA Repair and Meiotic Recombination in Mice. Wang X, Wang H, Guo B, Zhang Y, Gong Y, Zhang C, Xu H*, Wu X*. DNA Cell Biol. 2016 Oct;35(10):585-590. (*通讯作者)

    7. New congenital anomalies of the kidney and urinary tract and outcomes in Robo2 mutant mice with the inserted piggyBac transposon. Liu J, Sun L, Shen Q, Wu X, Xu H. BMC Nephrol. 2016 Jul 26;17(1):98.

    8. Generation of a Mouse Full-length Balancer with Versatile Cassette-shuttling Selection Strategy. Ye Z, Sun L, Li R, Han M, Zhuang Y, Wu X*, Xu T*. Int J Biol Sci. 2016 Jun 7;12(8):911-6. (*通讯作者)

    9. Lmod2 piggyBac mutant mice exhibit dilated cardiomyopathy. Li S, Mo K, Tian H, Chu C, Sun S, Tian L, Ding S, Li TR, Wu X, Liu F, Zhang Z, Xu T, Sun LV. Cell Biosci. 2016 June 4;6:38.

    10. Disruption of the Golgi protein Otg1 gene causes defective hormone secretion and aberrant glucose homeostasis in mice. Wang G, Li R, Yang Y, Cai L, Ding S, Xu T, Han M, Wu X*. Cell Biosci. 2016 Jun 10;6:41.. (*通讯作者)

    11. NADP(+)-IDH Mutations Promote Hypersuccinylation that Impairs Mitochondria Respiration and Induces Apoptosis Resistance. Li F, He X, Ye D, Lin Y, Yu H, Yao C, Huang L, Zhang J, Wang F, Xu S, Wu X, Liu L, Yang C, Shi J, He X, Liu J, Qu Y, Guo F, Zhao J, Xu W, Zhao S. Mol Cell. 2015 Nov 19;60(4):661-75.

    12. Leiomodin-3-deficient mice display nemaline myopathy with fast-myofiber atrophy. Tian L, Ding S, You Y, Li TR, Liu Y, Wu X, Sun L, Xu T. Dis Model Mech. 2015 Jun;8(6):635-41.

    13. Generation of genetically engineered mice by the piggyBac transposon system. Ding S, Xu T, Wu X*. Methods Mol Biol. 2014;1194:171-85. (*通讯作者)

    14. Rag GTPases are cardioprotective by regulating lysosomal function. Kim YC, Park HW, Sciarretta S, Mo JS, Jewell JL, Russell RC, Wu X, Sadoshima J, Guan KL. Nat Commun. 2014 Jul 1;5:4241.

    15. Rhbdd3 controls autoimmunity by suppressing the production of IL-6 by dendritic cells via K27-linked ubiquitination of the regulator NEMO. Liu J, Han C, Xie B, Wu Y, Liu S, Chen K, Xia M, Zhang Y, Song L, Li Z, Zhang T, Ma F, Wang Q, Wang J, Deng K, Zhuang Y, Wu X, Yu Y, Xu T, Cao X. Nat Immunol. 2014 Jul;15(7):612-622.

    16. Beadex affects gastric emptying in Drosophila. Ren J, Zhu H, Chi C, Mehrmohamadi M, Deng K, Wu X*, Xu T*. Cell Res. 2014 May;24(5):636-9. (*通讯作者)

    17. A piggyBac insertion disrupts Foxl2 expression that mimics BPES syndrome in mice. Shi F, Ding S, Zhao S, Han M, Zhuang Y, Xu T, Wu X*. 
Hum Mol Genet. 2014 Jul 15;23(14):3792-800. (*通讯作者)

    18. Histone methyltransferase ash1l suppresses interleukin-6 production and inflammatory autoimmune diseases by inducing the ubiquitin-editing enzyme a20. Xia M*, Liu J*, Wu X*, Liu S, Li G, Han C, Song L, Li Z, Wang Q, Wang J, Xu T, Cao X. Immunity. 2013 Sep 19;39(3):470-81. (*共同作者)

    19. piggyBac as a high-capacity transgenesis and gene-therapy vector in human cells and mice. Li R, Zhuang Y, Han M, Xu T*, Wu X*. Dis Mod Mech. 2013 May-Jun;6(3):828-33. (*通讯作者)

    20. Generation of Dhx9-deficient clones in T-cell development with a mitotic recombination technique. Zhu Y, Liu S, Yin Q, Xu T, Wu X*, Zhuang Y*. Genesis. 2012 Jul;50(7):543-51. (*通讯作者)

    21. Modeling Sjögren's syndrome with Id3 conditional knockout mice. Guo Z, Li H, Han M, Xu T, Wu X*, Zhuang Y*. Immunol Lett. 2011 Mar;135(1-2):34-42. (*通讯作者)

    22. Adam10 is essential for early embryonic cardiovascular development. Zhang C, Tian L, Chi C, Wu X, Yang X, Han M, Xu T, Zhuang Y, Deng K. Dev Dynam. 2010 Oct;239(10):2594-602.

    23. Endothelial SUR-8 acts in an ERK-independent pathway during atrioventricular cushion development. Yi J, Chen M, Wu X, Yang X, Xu T, Zhuang Y, Han M, Xu R. Dev Dynam. 2010 Jul;239(7):2005-13.

    24. Disruption of lysosome function promotes tumor growth and metastasis in Drosophila. Chi C, Zhu H, Han M, Zhuang Y, Wu X*, Xu T*. J Biol Chem. 2010 Jul;285(28):21817-23. (*通讯作者)

    25. SUN1/2 and Syne/Nesprin-1/2 complexes connect centrosome to the nucleus during neurogenesis and neuronal migration in mice. Zhang X, Lei K, Yuan X, Wu X, Zhuang Y, Xu T, Xu R, Han M. Neuron. 2009 Oct 29;64(2):173-87.

    26. C-terminal deletion of the atrophin-1 protein results in growth retardation but not neurodegeneration in mice. Yu J, Ying M, Zhuang Y, Xu T, Han M, Wu X*, Xu R*. Dev Dynam. 2009 Oct;238(10):2471-8. (*通讯作者)

    27. A large-scale functional approach to uncover human genes and pathways in Drosophila. Xu R, Deng K, Zhu Y, Wu Y, Ren J, Wan M, Zhao S, Wu X, Han M, Zhuang Y, Xu T. Cell Res. 2008 Nov;18(11):1114-27. (封面论文)

    28. ADAM10 is essential for proteolytic activation of Notch during thymocyte development. Tian L, Wu X, Chi C, Han M, Xu T, Zhuang Y. Int Immunol. 2008 Sep;20(9):1181-7.

    29. A mitotic recombination system for mouse chromosome 17. Sun L, Wu X*, Han M, Xu T*, Zhuang Y*. Proc Natl Acad Sci U S A. 2008 Mar 18;105(11):4237-41. (*通讯作者)

    30. PBmice: an integrated database system of piggyBac (PB) insertional mutations and their characterizations in mice. Sun LV, Jin K, Liu Y, Yang W, Xie X, Ye L, Wang L, Zhu L, Ding S, Su Y, Zhou J, Han M, Zhuang Y, Xu T, Wu X, Gu N, Zhong Y. Nucleic Acids Res. 2008 Jan;36(Database issue):D729-34.

    31. 哺乳动物PB 转座因子研究. 吴晓晖.《生命科学》2007, 19(2):114-6.

    32. 《实验小鼠》孙璘,吴晓晖等译. 高等教育出版社,2007,图字:01-2007-4094号,17万字。

    33. Muscle atrophy in transgenic mice expressing a human TSC1 transgene. Wan M, Wu X*, Guan KL, Han M, Zhuang Y, Xu T. FEBS Lett. 2006 Oct 16;580(24):5621-7. (*通讯作者)

    34. Sodium butyrate ameliorates histone hypoacetylation and neurodegenerative phenotypes in a mouse model for DRPLA. Ying M, Xu R, Wu X, Zhu H, Zhuang Y, Han M, Xu T. J Biol Chem. 2006 May 5;281(18):12580-6.

    35. The KASH domain protein MSP-300 plays an essential role in nuclear anchoring during Drosophila oogenesis. Yu J, Starr DA, Wu X, Parkhurst SM, Zhuang Y, Xu T, Xu R, Han M. Dev Biol. 2006 Jan 15;289(2):336-45. (封面论文)

    36. Efficient transposition of the piggyBac (PB) transposon in mammalian cells and mice. Ding S, Wu X*, Li G, Han M, Zhuang Y, Xu T*. Cell. 2005 Aug 12;122(3):473-83. (封面论文, *通讯作者)

    37. Ubiquitous expression of mRFP1 in transgenic mice. Zhu H, Wang G, Li G, Han M, Xu T, Zhuang Y*, Wu X*. Genesis. 2005 Jun;42(2):86-90. (*通讯作者)

    38. Overexpression of Human Genes in Drosophila melanogaster by Using GAL4 UAS System. Ma XZ, Cai LJ, Wu XH, Zhao SY, Li CB, Deng KJ. Acta Biochimica et Biophysica Sinica 2003, 35(7):597-600.

    39. “睡美人” 转座系统研究进展. 丁昇, 吴晓晖. 生物化学与生物物理进展 2003;30(1):43-8.

    40. Molecular cloning of a novel bovine homologue of the Drosophila tumorsuppressor gene, lats. Lu G, Hao X, Wu X, Xu T, Chen W. High Technology Letters 2002, 8(2): 1-4.

    41. Mutation in pre-mRNA adenosine deaminase markedly attenuates neuronal tolerance to O2 deprivation in Drosophila melanogaster. Ma E, Gu XQ, Wu X, Xu T, Haddad GG. Journal of Clinical Investigation 2001, 107(6):685-93.

    42. 果蝇在癌症研究中的作用. 吴晓晖, Potter CJ, Turenchalk GS, 许田《干细胞和发育生物学》Pp 1-12 军事医学科学出版社 (2000)

    43. 神经发育过程中的细胞间相互作用. Rooke, J. E., 吴晓晖, 许田.《细胞调控的探索——细胞信号传导、细胞凋亡和基因调控》 Pp 30-5 军事医学科学出版社 (1999).

    44. Processing of the notch ligand delta by the metalloprotease Kuzbanian, Qi H, Rand MD, Wu X, Sestan N, Wang W, Rakic P, Xu T, Artavanis-Tsakonas S. Science 1999, 283:91-4.

    45. Linkage analysis of the genetic markers of the candidate genes with non-insulin dependent diabetes mellitus in Chinese families. Ma L, Wang H, Chen J, He R, Wu X, Chai J. Chin Med J (Engl). 1998 Oct;111(10):895-8.

    46. Construction and physical mapping of the 35cM YAC contigs for the region of Xp11.2-p21.3. Wei Y, Wu XJ, Miao WM, Cai T, Wu XH, Kang YB, Chai JH. Acta Biochimica et Biophysica Sinica. 1997;29(1):98-106

    47. 非胰岛素依赖型糖尿病的遗传分析. 吴晓晖, 柴建华. 《生物工程进展》 1996;16(4):7-11.

    48. A 4.3 Mb YAC contig in Xp11.2: Long-range restriction mapping and identification of CpG islands. Wei Y, Cai T, Wu XH, Kang YB, and Chai JH. Acta Biochimica et Biophysica Sinica 1996;28(4):325-34.

    49. Construction and large-scale mapping of a 3.5-Mb YAC contig spanning human X chromosome Xp21.1-21.3. Wei Y, Cai T, Wu XH, Kang YB, and Chai JH. Acta Biochimica et Biophysica Sinica 1995;27(6):636-42.

    50. 哺乳动物人工染色体MAC-基因治疗的新载体. 康毅滨, 吴晓晖, 魏勇, 柴建华. 《生物工程进展》1995;15(4):18-21.

    51. Methylation of CpG islands and gene regulation. Kang, Y., Wu, X., Wei, Y., and Chai, J. (1995). Progress in Biochemistry and Biophysics 22(4): 300-3.

    52. 神经系统遗传病及其基因治疗. 吴晓晖, 康毅滨, 魏勇, 柴建华. 《生命科学》 1995;7(1):16-20.

    53. 人X染色体2bA3-YAC克隆重叠群的构建及其与DMD-YAC的串联. 魏勇、蔡倜、吴晓晖、康毅滨、柴建华.《高技术通讯》1994;4(12):1-4.